Composite

Part:BBa_K1074010:Design

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-25)


Pgrac+RBS+SDP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 772
    Illegal PstI site found at 2199
    Illegal AgeI site found at 959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 946


Design Notes

no


Source

Promoter Pgrac and RBS are synthesized via PCR, SDP operon is from genome of Bacillus subtilis

References